Research Paper Volume 18 pp 213—233

ATF5 regulates the expression of UPS, Autophagy-Lysosome System and other atrophy markers basally and with age. (A) Immunoblots of p53, Atrogin-1, MuRF1, 20S Proteasome subunit PSMB4, GADD45α, p21, Phosphorylated 4E-BP1 and Total 4E-BP1, corrected to Ponceau, with respective quantifications (B–H). Immunoblots of ATG7, V-ATPase, Parkin, Cathepsin B (mature (37kDa) and immature (50 kDa) and LC3I/II, with respective quantifications, corrected to Ponceau (I–M). Mature Cathepsin B was overexposed (long exposure: red saturation) in order to detect the levels of immature Cathepsin B. Mature Cathepsin B was quantified from the unsaturated/normal exposure immunoblot. The approximate molecular weights of proteins are indicated with a hash bar in kilodaltons (kDa). (n=4-7/group). A¶ P<0.05, A¶¶ P<0.01, A¶¶¶ P<0.001, main effect of age; G¶ P<0.05, main effect of genotype; AxG¶ P<0.05, interaction between age and genotype, Two-way ANOVA; Tukey’s post-hoc analyses, Young WT vs. Young ATF5 KO, Aged WT vs. Aged ATF5 KO, Young WT vs. Aged WT, Young ATF5 KO vs. Aged ATF5 KO, Young ATF5 KO vs. Aged WT, Young WT vs. Aged ATF5 KO. Tukey’s post-hoc: *P<0.05, **P<0.01. Y, Young; A, Aged. Data are means ± SD.