Research Paper Volume 18 pp 213—233
ATF5 is required for the maintenance of mitochondrial homeostasis and skeletal muscle health during aging
The normal expression of ISR/UPRmt and antioxidant markers are partially blunted in the absence of ATF5. (A) Representative immunoblots of LonP, mtHSP70, HSP60, corrected to mitochondrial content (MTCO1, blot from Figure 5A), and ATF4, ClpP, CHOP, Catalase, NQO1, MnSOD2 corrected to Ponceau, with respective quantifications (B–J). The approximate molecular weights of proteins are indicated with a hash bar in kilodaltons (kDa). (n=4-7/group). A¶ P<0.05, A¶¶ P<0.01, main effect of age; G¶ P<0.05, G¶¶ P<0.01 main effect of genotype, Two-way ANOVA. Y, Young; A, Aged. Data are means ± SD.