Research Paper Volume 18 pp 213—233

RNA-Seq analysis of young ATF5 KO mice relative to WT counterparts. (A) Volcano plot of significantly differential genes in WT vs. ATF5 KO mice. Genes which had abs(Log2FC) > 1 (=2x FC) and padj (FDR) < 0.05, where colour red or blue depending on the directional fold-change. (B) Gene Ontology (GO) Enrichment Analysis of Upregulated genes in ATF5 KO’s relative to WT’s. Classifications of genes were based of sorting into biological process (BP), Cellular Component (CC) and Molecular Function) with gene count size and enchainment score displayed. Heat map of DESeq2 RNA-Seq data showing differential expression of (C) Upregulated and (D) Downregulated genes in ATF5 KO vs. WT mice. Mitochondrial stress-related genes were not differentially altered. The data are expressed as log2 fold change from ATF5 KO vs. WT (enhanced gene expression, red; reduced gene abundance, blue; and no change in gene expression, white; FDR < 0.05 and padj <0.05 see Supplementary File 1 for expanded DESeq2 analysis data table.