Figure 1.Lung Mtb burden remains similar till 6 weeks post-infection across age groups and sexes in C57BL/6 mice. (A) Schematic of the experimental design used for aerosol Mtb H37Rv infection of C57BL/6 mice [female (♀) and male (♂)] across age groups. (B) Changes in body weight of mice groups (healthy: H, ….; infected: I, ___) are presented, n=5-10/age group/condition; dashed vertical line represents day of infection. (C) Lung bacterial burden (in log10cfu/mL) in mice post Mtb infection; n=5-10/timepoint/age group/condition; dashed horizontal line represents limit of detection (LOD). Young (2-4 months) in blue, middle-aged (9-12 months) in green and old (17-19 months) mice in brown; cfu= colony forming unit; w.p.i.= weeks post infection; p-value: ** <0.005 at 95% confidence interval by Mann-Whitney test. Data shown as mean ± SD. See also Supplementary Figures 1, 2.
