Figure 5.Effect of aging and 753b treatment on aggrecan protein fragmentation in mouse IVD tissues. Western blots for aggrecan in total protein of whole IVDs isolated from five (A) male and (B) female animals. Quantitation of the Western blot results of (C) male and (D) female for aggrecan and MMP-generated fragments in total protein of IVDs by the Bio-Rad GelDoc. a, ADAMTS-generated aggrecan fragments; m, MMP-generated aggrecan fragments.
