Figure 4.Loss of fzo-1, unc-43, or daf-16 abrogates mitochondrial network preservation in prx-11-RNAi animals. (A) Representative images of Mito-GFP in body wall muscle of day 5 and day 10 adult wild-type, fzo-1, unc-43, and daf-16 hermaphrodite animals fed either control or prx-11 RNAi. (B) Quantification of mitochondrial junctions per object in day 5 and day 10 adult wild-type, fzo-1, unc-43, and daf-16 hermaphrodite animals fed either control or prx-11 RNAi (n = 10 worms per condition). Data are presented as mean ± SD. ****, p ≤ 0.0001; ns, not significant. One-way ANOVA with Tukey’s multiple comparisons. (C) Mito-GFP object lengths in day 5 and day 10 adult wild-type, fzo-1, unc-43, and daf-16 hermaphrodite animals fed either control or prx-11 RNAi (n = 10 worms per condition). Data are presented a box-and-whisker plots (minimum, 25th percentile, median, 75th percentile, maximum). ****, p ≤ 0.0001; ***, p ≤ 0.001; **, p ≤ 0.01; ns, not significant. One-way ANOVA with Tukey’s multiple comparisons. (D) Representative image of DAF-16::GFP in side-by-side day 5 adult hermaphrodite animals fed either control or prx-11 RNAi. Nuclei are encircled by dashed lines in the insets. (E) Quantification of nuclear:cytoplasmic DAF-16::GFP fluorescence ratios in day 5 adult hermaphrodite animals fed either control or prx-11 RNAi (n = 15 worms per condition). Data are presented as mean ± SD. ****, p ≤ 0.0001. Unpaired t-test. Bars: 10 μm.
