Figure 6.fzo-1, unc-43, and daf-16 mutations block lifespan extension upon prx-11 knockdown. (A) Lifespan analysis of wild-type and fzo-1 hermaphrodite animals fed either control or prx-11 RNAi. Wild-type control: Mean lifespan = 17.47 days, n = 184 worms. Wild-type prx-11 RNAi: Mean lifespan = 19.99 days, n = 195 worms. fzo-1 control: Mean lifespan = 16.08 days, n = 171 worms. fzo-1 prx-11 RNAi: Mean lifespan = 16.07 days, n = 162 worms. Significance was determined using a log-rank test. Wild-type control vs. wild-type prx-11 RNAi: Χ2 = 34.61, p ≤ 0.0001; fzo-1 control vs. fzo-1 prx-11 RNAi: Χ2 = 0.01, ns, not significant. (B) Lifespan analysis of wild-type and unc-43 hermaphrodite animals fed either control or prx-11 RNAi. Wild-type control: Mean lifespan = 18.94 days, n = 192 worms. Wild-type prx-11 RNAi: Mean lifespan = 21.37 days, n = 196 worms. unc-43 control: Mean lifespan = 17.38 days, n = 193 worms. unc-43 prx-11 RNAi: Mean lifespan = 17.77 days, n = 182 worms. Significance was determined using a log-rank test. Wild-type control vs. wild-type prx-11 RNAi: Χ2 = 31.86, p ≤ 0.0001; unc-43 control vs. unc-43 prx-11 RNAi: Χ2 = 0.03, ns, not significant. (C) Lifespan analysis of wild-type and daf-16 hermaphrodite animals fed either control or prx-11 RNAi. Wild-type control: Mean lifespan = 17.75 days, n = 194 worms. Wild-type prx-11 RNAi: Mean lifespan = 20.21 days, n = 178 worms. daf-16 control: Mean lifespan = 14.56 days, n = 189 worms. daf-16 prx-11 RNAi: Mean lifespan = 14.55 days, n = 194 worms. Significance was determined using a log-rank test. Wild-type control vs. wild-type prx-11 RNAi: Χ2 = 24.21, p ≤ 0.0001; daf-16 control vs. daf-16 prx-11 RNAi: Χ2 = 0.13, ns, not significant.
