Figure 3.TM4SF1 transcription and protein expression increase with HFD and are decreased following senolytic treatment. (A) RNA-seq heat map showing elevated Tm4sf1 expression in high fat diet (HFD) and decreased with a BCL2i (ABT263) which acts as a senolytic. Plaur and Tm4sf1 followed a similar pattern to Cdkn1a and Bcl2, senescence markers. n=4 Control, n=4 HFD, n=4 HFD + Bcl2i. (B) Representative immunostaining of mouse pancreas for TM4SF1, P21, and insulin, consistent with increased expression in HFD and reduction with BCL2i treatment. (C) TM4SF1 and (D) MFI over the total islet area, normalized to cell count per islet, showing increased expression in HFD and reduction with BCL2i treatment. n=3 Control, n=3 HFD, n=3HFD + Bcl2i. 10-13 images per animal were analyzed.
