Figure 7.TM4SF1 levels negatively correlate with β-cell function and insulin content in human islets. Negative linear regression between stimulation index (1→10 mM) and PLAUR transcript (A) and TM4SF1 transcript (B). Scatter plots showing the relationship between insulin content after culture and transcript levels of PLAUR (C) and TM4SF1 (D) in human islets. Data downloaded from HumanIslets.com. (E) Experimental design to evaluate function from uPAR and TM4SF1 subpopulations. (F) Glucose-stimulated insulin secretion (16.8 mM glucose) in UPAR+ and TM4SF1+ β-cells normalized to their negative counterparts, showing significantly reduced insulin secretion in TM4SF1+ cells; n= 5 technical replicates from Donor 4.
