Research Paper Volume 11, Issue 9 pp 2836—2851
Hepatic histopathology and apoptosis in diet-induced-obese mice under Escherichia coli pneumonia
- 1 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China
- 2 Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, Sichuan 611130, PR China
- 3 School of Public Health, Chengdu Medical College, Chengdu, Sichuan 610500, PR China
received: January 13, 2019 ; accepted: May 3, 2019 ; published: May 14, 2019 ;https://doi.org/10.18632/aging.101956
How to Cite
Copyright: Song et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This research was to investigate the difference of hepatic histopathology and apoptosis between the diet-induced obesity (DIO) and normal (lean) mice after Escherichia coli (E. coli) pneumonia. A total of 128 ICR mice were selected to be challenged intranasally with phosphate-buffered saline (PBS) or 4×109CFUs/mL of E. coli, and the liver histopathology and apoptosis were examined pre- and post-infection. Results showed that the liver index, levels of lipid droplets, cytokines, adipocytokines, oxidative stress, apoptotic percentage, and apoptotic related factors in the E. coli-infected mice were generally higher than those in the uninfected mice, whereas the hepatic glycogen and Bcl-2 were the opposite. Interestingly, after E. coli infection, the DIO-E. coli mice exhibited decreased liver index and apoptotic percentages, and reduced levels of TNF-α, IL-6, resistin, MDA, GSH, CAT, Caspase-3, Caspase-9, Bax as well as Bax/Bcl-2 ratio in comparison to the lean-E. coli mice. Our results indicated that E. coli-induced pneumonia caused hepatic histopathological damage, increased hepatic apoptosis, oxidative damages, and higher levels of cytokines and adipocytokines. However, such changes showed less severely in the DIO mice than in the lean mice following E. coli pneumonia.