Research Paper Volume 12, Issue 1 pp 718—739
Integrated transcriptome expression profiling reveals a novel lncRNA associated with L-DOPA-induced dyskinesia in a rat model of Parkinson’s disease
- 1 Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China
- 2 Beijing Key Laboratory of Neurostimulation, Beijing, China
- 3 Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- 4 Department of Pathology, School of Basic Medical Sciences, Capital Medical University, Beijing, China
- 5 Department of Pathophysiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China
Received: August 29, 2019 Accepted: December 24, 2019 Published: January 10, 2020
https://doi.org/10.18632/aging.102652How to Cite
Abstract
Levodopa-induced dyskinesia (LID) is a common complication of chronic dopamine replacement therapy in the treatment of Parkinson’s disease (PD). Long noncoding RNAs regulate gene expression and participate in many biological processes. However, the role of long noncoding RNAs in LID is not well understood. In the present study, we examined the lncRNA transcriptome profile of a rat model of PD and LID by RNA sequence and got a subset of lncRNAs, which were gradually decreased during the development of PD and LID. We further identified a previously uncharacterized long noncoding RNA, NONRATT023402.2, and its target genes glutathione S-transferase omega (Gsto)2 and prostaglandin E receptor (Ptger)3. All of them were decreased in the PD and LID rats as shown by quantitative real-time PCR, fluorescence in situ hybridization and western blotting. Pearson’s correlation analysis showed that their expression was positively correlated with the dyskinesia score of LID rats. In vitro experiments by small interfering RNA confirmed that slicing NONRATT023402 inhibited Gsto2 and Ptger3 and promoted the inflammatory response. These results demonstrate that NONRATT023402.2 may have inhibitive effects on the development of PD and LID.