Research Paper Volume 13, Issue 12 pp 16485—16499
Cell landscape atlas for patients with chronic thromboembolic pulmonary hypertension after pulmonary endarterectomy constructed using single-cell RNA sequencing
- 1 Medical Research Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 2 Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Institute of Respiratory Medicine, Beijing 100020, China
- 3 Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
- 4 Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 5 Department of Echocardiography, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 6 Department of Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 7 Department of Interventional Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 8 Department of Pathology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
- 9 Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China
- 10 Department of Radiology, China-Japan Friendship Hospital, Beijing 100029, China
- 11 Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
Received: December 16, 2020 Accepted: May 13, 2021 Published: June 21, 2021
https://doi.org/10.18632/aging.203168How to Cite
Copyright: © 2021 Miao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
This study aimed to construct an atlas of the cell landscape and comprehensively characterize the cellular repertoire of the pulmonary endarterectomized tissues of patients with chronic thromboembolic pulmonary hypertension (CTEPH). Five pulmonary endarterectomized tissues were collected. 10× Genomics single-cell RNA sequencing was performed, followed by the identification of cluster marker genes and cell types. Gene Ontology (GO) enrichment analysis was conducted. Seventeen cell clusters were characterized, corresponding to 10,518 marker genes, and then classified into eight cell types, including fibroblast/smooth muscle cell, endothelial cell, T cell/NK cell, macrophage, mast cell, cysteine rich secretory protein LCCL domain containing 2 (CRISPLD2)+ cell, cancer stem cell, and undefined. The specific marker genes of fibroblast/smooth muscle cell, endothelial cell, T cell/NK cell, macrophage, mast cell, and cancer stem cell were significantly enriched for multiple functions associated with muscle cell migration, endothelial cell migration, T cell activation, neutrophil activation, erythrocyte homeostasis, and tissue remodeling, respectively. No functions were significantly enriched for the marker gene of CRISPLD2+ cell. Our study, for the first time, provides an atlas of the cell landscape of the pulmonary endarterectomized tissues of CTEPH patients at single-cell resolution, which may serve as a valuable resource for further elucidation of disease pathophysiology.