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Research Paper|Volume 13, Issue 15|pp 19878—19893

CCL2 associated with CD38 expression during ex vivo expansion in human cord blood-derived hematopoietic stem cells

Chao-Ling Yao1,2,3, Poyin Huang4,5,6,7, Tsai-Chi Liu8, Yung-Kai Lin9,10, Ching-Yun Chen8, Yi-Ting Lai2, Tzu−Yun Chin8, Tsung-Yu Tseng3, Yi-Chiung Hsu8
  • 1Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan
  • 2Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City 320, Taiwan
  • 3Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan City 320, Taiwan
  • 4Department of Neurology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung City 807, Taiwan
  • 5Department of Neurology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, Kaohsiung City 807, Taiwan
  • 6Neuroscience Research Center, Kaohsiung Medical University, Kaohsiung City 807, Taiwan
  • 7Department of Neurology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City 807, Taiwan
  • 8Department of Biomedical Sciences and Engineering, National Central University, Chung-Li, Taoyuan City 320, Taiwan
  • 9Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung City 202, Taiwan
  • 10Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan
* Equal contribution
Received: February 22, 2021Accepted: July 8, 2021Published: August 10, 2021
https://doi.org/10.18632/aging.203398

Copyright: © 2021 Yao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

To date, different experimental strategies have been developed for the ex vivo expansion of human hematopoietic stem cells (HSCs) for clinical applications. However, differences in the genomic function of expanded HSCs under different culture systems remain unclear. In this study, we compared the gene expression profiles of HSCs in ex vivo expanded serum (10% FBS, fetal bovine serum) and serum-free culture systems and analyzed the molecular functions of differentially expressed genes using microarray chips. We identified 839 differentially expressed genes between the two culture systems. These genes were enriched in the TNF -regulated inflammatory pathway in an FBS culture system. In addition, the mRNA expression of CCL2 (C-C motif chemokine ligand 2), TNF (tumor necrosis factor) and FOS (FBJ murine osteosarcoma viral oncogene homolog) was validated by RT-qPCR. Our data revealed that ex vivo expansion of HSCs using the FBS culture system induces an inflammatory response and high CD38 expression, indicating that this system might activate an inflammatory pathway and induce expression of the cancer marker CD38 during ex vivo expansion of HSCs. This study provides a transcriptional profile and new insights into the genomic functions of HSCs under different expanded cultures.