Research Paper Volume 13, Issue 21 pp 24402—24416

miR-6745-TIMP1 axis inhibits cell growth and metastasis in gastric cancer

Hui Liu1, *, , Yuan Xiang1,2, *, , Qi-Bei Zong1, *, , Xiao-Yu Zhang1, , Zhi-Wen Wang3, , Shi-Qiang Fang2, , Tong-Cun Zhang1, , Xing-Hua Liao1, ,

  • 1 Institute of Biology and Medicine, College of Life and Health Sciences, Wuhan University of Science and Technology, Hubei 430081, P.R. China
  • 2 Department of Medical Laboratory, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430014, P.R. China
  • 3 Yueyang Key Laboratory of Chronic Noncommunicable Diseases, Yueyang Vocational and Technical College, Hunan 414000, P.R. China
* Equal contribution

Received: August 6, 2021       Accepted: October 27, 2021       Published: November 14, 2021
How to Cite

Copyright: © 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Tissue inhibitor matrix metalloproteinase 1 (TIMP1) has been reported to act as a tumor oncogene in colon cancer. However, little is known about the biological role of TIMP1 in gastric cancer. In this study, we found that the expression of TIMP1 in GC tissues was upregulated compared with the normal gastric tissues. TIMP1 was confirmed as a direct target of miR-6745 and silencing TIMP1 mimicked the effects of miR-6745 in GC cells. Further mechanism studies have shown that miR-6745 inhibits the Wnt/β-catenin pathway by targeting TIMP1, thereby inhibiting cell proliferation, migration and invasion. In addition, through the analysis of GC tissues, a negative correlation between miR-6745 and TIMP1 was found in 42 GC tissues. Our findings indicate that the miR-6745-TIMP1 axis regulates Wnt/βcatenin signaling and participates in GC tumorigenesis and provide a potential therapeutic target for preventing GC progression.


IOD: integrated optical density; UTR: untranslated region; GC: gastric cancer.