Research Paper
Pachymic acid ameliorates myocardial ischemia and reperfusion injury by attenuating cardiomyocyte apoptosis through the regulation of the mTOR signal pathway
- 1 Cadre Diagnosis and Treatment Department, Eighth Medical Center of PLA General Hospital, Beijing 100091, China
- 2 Department of Cardiovascular, The First Medical Center of PLA General Hospital, Beijing 100853, China
- 3 Department of Pharmacy, Medical Supplies Center of Chinese PLA General Hospital, Beijing 100853, China
- 4 Foreign Languages College, Guilin University of Electronic Technology, Guilin 541004, Guangxi, China
- 5 Personnel Department, Fuxin Center Hospital, Fuxin 123000, Liaoning, China
- 6 Department of Cardiac Function, Fuxin Center Hospital, Fuxin 123000, Liaoning, China
Received: April 6, 2021 Accepted: August 23, 2021
https://doi.org/10.18632/aging.203693How to Cite
Copyright: © 2021 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
To explore the function and mechanism of Pachymic acid (PA) on myocardial ischemia /reperfusion. TUNEL, flow cytometry, and western blot were performed to detect the apoptosis level in neonatal mice ventricular cells (NMVCs). ATP, ROS, mPTP, JC-1, GSH, GR activity levels were assessed to evaluate the mitochondrial function. Autophagy level was detected by p-mTOR and LC3II fluorescence intensity and autophagy-associated protein level. Cardiac function was determined by Ejection fraction (EF) and shortening fraction (FS). PA inhibited the apoptosis level and recovered the mitochondrial function in NMVCs after hypoxia/reperfusion. Moreover, PA markedly inhibited ischemia-reperfusion (I/R)-induced autophagy by inhibiting the protein and mRNA levels of LC3-II, Beclin1, ATG5, and ATG7 and the number of autophagosomes and by involving the mTOR pathway. An increasing EF and FS indicated the improved cardiac function, and decreasing oxidative stress was found in PA treated group. The results showed that PA blocked excessive autophagy and apoptosis in I/R mice heart or H/R NMVCs, which was mediated by activating the mTOR pathway.