Research Paper Volume 14, Issue 14 pp 5628—5640

Short-term resveratrol treatment restored the quality of oocytes in aging mice

Naoki Okamoto1,2, , Yorino Sato1,2,3, , Yuta Kawagoe1,2,3, , Takahiko Shimizu4, , Kazuhiro Kawamura1,3, ,

  • 1 Department of Obstetrics and Gynecology, International University of Health and Welfare School of Medicine, Chiba 286-8686, Japan
  • 2 Department of Biodesign, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062, Japan
  • 3 Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan
  • 4 Aging Stress Response Project Team, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan

Received: October 12, 2021       Accepted: June 23, 2022       Published: July 8, 2022
How to Cite

Copyright: © 2022 Okamoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The quality of oocytes declines by aging, resulting in their low competences for fertility. Here, resveratrol treatment showed increases in the rates of implantation and live offspring as well as decreases in the abortion rate as short as one week after treatment, although the number of ovulated oocytes and the rates of fertilization and blastocyst formation were not changed following resveratrol treatment. Resveratrol treatment did not cause abnormalities mouse estrous cycles and body weights. No abnormality was detected in both fetuses and placentas after 22 weeks of resveratrol treatment and the fetuses had normal fertility. Positive correlations were found between serum resveratrol levels and pregnancy and live offspring rates as well as ovarian expression levels of Sirt1, Sirt3, Sirt4, Sirt5, and Sirt7. The mitochondrial membrane potential and ATP content but not copy number of mitochondrial DNA in oocytes was increased in aging mice with resveratrol treatment. In conclusion, we demonstrated the restoration of oocyte quality in aging mice in addition to the prevention of their quality decline during aging by restoring mitochondrial functions by resveratrol treatment without adverse effects in the animals and their offspring.


IVF-ET: In vitro fertilization and embryo transfer; HPLC: high performance liquid chromatography; MS/MS: tandem mass spectrometry; RD: resveratrol diet; CD: control diet; COCs: cumulus oocyte complexes; Res: resveratrol; wks: weeks.