Research Paper Volume 8, Issue 9 pp 2100—2126

Figure 2. Cell cycle genes function in the germline longevity pathway. (A) cye-1(RNAi) does not alter longevity of germline-defective glp-1(e2141) mutants. glp-1 mutants were raised at 25°C until L4/early adulthood to eliminate germ cells and then shifted to 20°C for the rest of the assay. (B) Lifespans of germline-defective mes-1 mutants. mes-1(bn7) induced sterility is approximately 50% penetrant at 20°C [7]. The sterile and fertile mes-1 animals were identified by their appearance using a dissecting microscope. cye-1(RNAi) extends lifespan in fertile mes-1 animals but not in sterile mes-1 mutants. (C) The steroid hormone signaling pathway is required for cye-1(RNAi) longevity. The nuclear hormone receptor daf-12(rh61rh411) mutants greatly suppress the longevity of cye-1(RNAi) treated worms. (D) Inactivation of the steroid hormone biosynthesis enzyme daf-9 abolishes lifespan extension caused by cye-1(RNAi). Survival plots show combined data from at least two experiments. Quantitative data and statistical analyses of lifespan assays are shown in Table 1, individual experiments are presented in Table S1. EV refers to empty control vector.