Research Paper Volume 10, Issue 4 pp 622—631

Stress resistance and lifespan enhanced by downregulation of antimicrobial peptide genes in the Imd pathway

Figure 4. Stress resistance and lifespan enhanced by downregulation of AMP genes in Imd pathway. (A) Gene expressions of AttC and loco in AttC-P homozygous mutant, which were normalized with those of wild-type (+/+: 1 fold) using total RNAs of 2-day-old male flies developed at 25°C. (B) The survival curve for oxidation stress using 2-day-old male flies (p-value: log-rank test). (C) Increased stress resistance of 2-day-old AttC-P mutant flies from the wild-type flies (+/+: 0%). The median survival times of flies under each stress (starvation, oxidation or heat) were calculated from the several survival curves (B) and then, the percentages changed from wild-type flies are represented as average ± SEM. (D) The lifespan of adult male flies between the wild-type and AttC-P mutant. Parenthesis: increased percentage of mean lifespan of AttC-P mutant from the wild-type flies. (E) The survival curve for heat stress using 2-day-old male flies between single transgene controls (+/armG4, DptBRi/+) and DptB downregulation in a whole body (DptBRi/armG4). (F) Increased stress resistance of 2-day-old DptBRi/armG4 flies from the control +/armG4 (0%). Parenthesis: increased percentage of median survival time of the DptBRi/armG4 flies from another control DptBRi/+. (G) The lifespan of adult male flies between the two controls and DptB downregulation. Parenthesis: increase percentage of DptBRi/armG4 flies from the DptBRi/+ control.