Research Paper Volume 10, Issue 4 pp 645—657

AVE0991, a nonpeptide analogue of Ang-(1-7), attenuates aging-related neuroinflammation

Figure 2. Ang-(1-7) levels are decreased in the brains of SAMP8 mice during the aging process. (A) The activity of ACE2 in the brains of 4-, 8-, and 12-month-old SAMP8 mice as well as their age-matched SAMR1 control mice were assessed using a specific detection kit. (B) The Ang-(1-7) levels in the brains of 4-, 8-, and 12-month-old SAMP8 mice as well as their age-matched SAMR1 control mice were detected by ELISA. (C) The expression of Mas1 mRNA levels in the brains of 4-, 8-, and 12-month-old SAMP8 mice as well as their age-matched SAMR1 control mice were assessed by qRT-PCR. Gapdh was used as an internal control. Data from panel B and C were expressed as a fold change relative to 4-month-old SAMR1 control mice. All data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Columns represent mean ± SD (n=8 per group). *P<0.05. #P<0.05 versus age-matched SAMR1 control mice.