Research Paper Volume 10, Issue 4 pp 645—657

AVE0991, a nonpeptide analogue of Ang-(1-7), attenuates aging-related neuroinflammation

Figure 3. AVE0991 attenuates neuroinflammation in the brains of SAMP8 mice. Eight-month-old SAMP8 mice were injected intraperitoneally with vehicle or AVE0991 (1, 3 or 10 mg/kg/day) for 30 days. Afterwards, mice were sacrificed for analysis. (A) The mRNA levels of Il1b in the brains were investigated by qRT-PCR. (B) The mRNA levels of Il6 in the brains were investigated by qRT-PCR. (C) The mRNA levels of Tnf in the brains were investigated by qRT-PCR. (D) The protein levels of IL-1β in the brains were investigated by ELISA. (E) The protein levels of IL-6 in the brains were investigated by ELISA. (F) The protein levels of TNF-α in the brains were investigated by ELISA. In panel A-C,Gapdh was used as an internal control, and data were expressed as a fold change relative to non-treated SAMP8 mice. All data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Columns represent mean ± SD (n=8 per group). #P<0.05 versus vehicle-treated SAMP8 mice.