Research Paper Volume 11, Issue 6 pp 1791—1803

Muscle-derived miR-34a increases with age in circulating extracellular vesicles and induces senescence of bone marrow stem cells

Figure 3. Hydrogen peroxide increases miR-34a in EVs secreted by C2C12 myoblasts and human myotubes, and these EVs can reduce bone stem cell (BMSC) viability and increase senescence. (A) EVs isolated from C2C12 cells treated with hydrogen peroxide show a significant increase in miR-34a. (B) EVs isolated from human myotubes treated with hydrogen peroxide show a significant increase in miR-34a. (C) BMSC viability indicated by MTT assay is significantly reduced after treatment with EVs isolated from these C2C12 cells exposed to hydrogen peroxide. (D) BMSC senescence measured by beta-galactosidase (β-gal) assay is significantly increased after treatment with EVs isolated from these C2C12 cells exposed to hydrogen peroxide. *P<.05, **P<.01.