Figure 5. Intermediate metabolites in pyrimidine metabolism influence the lifespan of C. elegans through regulating reproductive signals. (A–D) Lifespan analysis of glp-1 (e2144) animals treated with 0.5 mM (A) β-aminoisobutyrate (red), (B) cytidine (red), (C) orotate (red), (D) uridine (red), and water (black) on heat-inactivated E. coli OP50 (P value by log-rank test). The replicated data are summarized in Supplementary Table 1. (E) Quantification of ORO staining. Mean ± SD.; n ≥ 30 (Student’s t test). (F) DAPI-stained image of the gonads of N2 worms treated with 0.5 mM β-aminoisobutyrate, cytidine, orotate, and uridine and the untreated control. (G) Quantification of the germline stem cells in wild-type N2 worms treated with or without 0.5 mM of the metabolites. P values were calculated using Student’s t test.