Research Paper Volume 11, Issue 12 pp 3993—4010

Intermediate metabolites of the pyrimidine metabolism pathway extend the lifespan of C. elegans through regulating reproductive signals

Figure 5. Intermediate metabolites in pyrimidine metabolism influence the lifespan of C. elegans through regulating reproductive signals. (AD) Lifespan analysis of glp-1 (e2144) animals treated with 0.5 mM (A) β-aminoisobutyrate (red), (B) cytidine (red), (C) orotate (red), (D) uridine (red), and water (black) on heat-inactivated E. coli OP50 (P value by log-rank test). The replicated data are summarized in Supplementary Table 1. (E) Quantification of ORO staining. Mean ± SD.; n ≥ 30 (Student’s t test). (F) DAPI-stained image of the gonads of N2 worms treated with 0.5 mM β-aminoisobutyrate, cytidine, orotate, and uridine and the untreated control. (G) Quantification of the germline stem cells in wild-type N2 worms treated with or without 0.5 mM of the metabolites. P values were calculated using Student’s t test.