Research Paper Volume 11, Issue 14 pp 5232—5245

Aberrant DNA methylation of synaptophysin is involved in adrenal cortisol-producing adenoma

Figure 3. Different miRNA expression in CPA and normal adrenal tissues and the function of miR-27a-5p on the proliferation of H295R cells. (A) Hierarchical clustering of differentially expressed miRNAs in CPA samples and normal adrenal tissues. (B) miR-27a-5p is downregulated about 5.4-fold in CPA in comparison with normal adrenal cortex tissues as indicated by microarray analysis. (C) qRT-PCR analysis of miR-27a-5p in CPA samples and their adjacent normal adrenal cortex tissues. Three independent experiments were performed, and representative data are shown. (D) Upregulation of miR-27a-5p using miR-27a-5p mimics, or downregulation of miR-27a-5p using miR-27a-5p inhibitor in H295R cells were confirmed by qRT-PCR. (E) Cell viability in H295R cells transfected with miR-27a-5p mimics and inhibitor were determined by the CCK-8 assay. (FG) Analysis of EdU staining on miR-27a-5p mimic-treated H295R cells. The EdU incorporation rate was expressed as the ratio of EdU-positive cells to total DAPI positive cells. Red, EDU; Blue, DAPI. Magnification, 400×. Three independent experiments were performed, and representative data are shown. NC, normal control. **p<0.01. *p<0.05. NC, normal control.