Research Paper Volume 11, Issue 23 pp 11659—11672

Nickel induces inflammatory activation via NF-κB, MAPKs, IRF3 and NLRP3 inflammasome signaling pathways in macrophages

Figure 4. NiCl2 induces pro-inflammatory cytokine production in BMDMs. (A) The mRNA expression levels of inflammatory cytokines after NiCl2 (0, 0.1, 0.5 and 1.0 mM) treatment for 24h. (B) Inflammatory cytokine expression is quantified in BMDMs cultural medium by ELISA after NiCl2 (0, 0.1, 0.5 and 1.0 mM) treatment for 24h. Data are presented with the means ± standard deviation (n=5). *p < 0.05 and **p < 0.01, compared with the control group.