Figure 3. Aspirin increased the inhibiting effect of Cisplatin on cell migration and invasion in colon cancer cells. (A) A wound-healing assay of human colon cancer cells RKO and LoVo treated with Cisplatin alone or Aspirin alone or their combination at indicated dose for 56 h was performed to detect the capacity of cell migration. Scale bars, 500 μm. The cell migration rate was calculated through the quantification of migration distance (n=4). (B) The capacity of cell invasion of human colon cancer cells RKO and LoVo treated with Cisplatin alone or Aspirin alone or their combination at indicated dose for 96 h was analyzed by a transwell assay. The invaded cells were stained by crystal violet and the representative images were taken by inverted microscope (n=4). Scale bars, 200 μm. (C) The expression levels of main EMT- related proteins N-cadherin, β-catenin, Vimentin, MMP2, MMP9, Snail and Slug in human colon cancer cell RKO treated with Cisplatin alone (15 μM) or Aspirin alone (10 mM) or their combination for 48 h were detected by western blot assay (n=3). Data were presented as means ± SD, *P<0.05, **P<0.01, ***P<0.001.