Research Paper Volume 12, Issue 5 pp 4424—4444

Discovery and validation of the prognostic value of the lncRNAs encoding snoRNAs in patients with clear cell renal cell carcinoma

Figure 7. The validation of the expression patterns and the methylation status of SNHG3 and SNHG15. (A) q-RT-PCR analysis of SNHG3 and SNHG15 expression in ccRCC and adjacent normal renal tissues. (B) Patients were grouped according to the median cutoff of SNHG3 and SNHG15 expression for OS detection, patients were grouped according to the median cutoff of SNHG15 expression for RFS detection, and the correlation between SNHG3 and SNHG15 expression and the tumor stage of ccRCC patients. (C) Pyrosequencing analysis of cg07807470, cg15161854, cg00953154 and cg16459265 methylation levels between ccRCC and matched adjacent normal tissues. (D) The methylation levels of cg07807470 and cg15161854 were negatively associated with the expression of SNHG3. (E) The methylation levels of cg00953154 and cg16459265 were negatively associated with the expression of SNHG15. (F) Patients were grouped according to the median cutoff of cg15161854 and cg16459265 methylation status for OS detection. (G) Patients were grouped according to the median cutoff of cg15161854 and cg16459265 methylation status for RFS detection.