Research Paper Volume 12, Issue 5 pp 4506—4526

LncRNA GUSBP5-AS promotes EPC migration and angiogenesis and deep vein thrombosis resolution by regulating FGF2 and MMP2/9 through the miR-223-3p/FOXO1/Akt pathway

Figure 5. LncRNA GUSBP5-AS directly bound with miR-223-3p. (A) Bioinformatics analysis revealed GUSBP5-AS contains binding sequences complementary to the binding sites of miR-223-3p. (B) The luciferase reporter constructs containing WT-GUSBP5-AS or MU-GUSBP5-AS sequence. (C) Schematic graph of the constructed reporter plasmid containing putative. (D) Dual-luciferase reporter assay verified the targeting relationship between miR-223-3p and GUSBP5-AS. (E) The expression of miR-223-3p was measured by qRT-PCR after cells treated with GUSBP5-AS, GUSBP5-AS+miR-223-3p or miR-223-3p. (F) Effects of cotransfection of GUSBP5-AS and miR-223-3p on in vitro tube formation were analysed. Scale bar=100μm. (original magnification, ×100). (G) Effects of cotransfection of GUSBP5-AS and miR-223-3p on cell migration were detected by wound healing assay. Scale bar=100μm. (original magnification, ×100). (H) Effects of cotransfection of GUSBP5-AS and miR-223-3p on cell invasion were analysed by transwell cell invasion assay. Scale bar=100μm. (original magnification, ×100). **P < .01, ***P < .001 compared to the NC group, ###P < .001 compared to the GUABP5-AS treated group. Data are represented as mean ± SEM.