Research Paper Volume 12, Issue 9 pp 7747—7760

TGF-β signaling regulates SPOP expression and promotes prostate cancer cell stemness

Figure 2. SPOP is regulated by TGF-β Signaling in prostate cancer. (A) Western blot analysis the expression of SPOP in the oncospheres in androgen-independent (DU145, PC3) cell lines and androgen-dependent (LNCaP) cell lines. (B) Real-Time PCR analysis of SPOP and CSCs markers expression in adherent cells versus spheres in DU145 cells. Data are normalized to Actin expression and presented as fold change in gene expression relative to adherent cells. Data are means ± SEM (n=3). **P < 0.01 vs Adherent (Student's t-test). (C) Real-Time PCR analysis of SPOP and CSCs markers expression in adherent cells versus spheres in LNCaP cells. Data are normalized to Actin expression and presented as fold change in gene expression relative to adherent cells. Data are means ± SEM (n=3). **P < 0.01 vs Adherent (Student's t-test). (D) Analysis the expression of SPOP in the treatment of TGF-β (10ng/ml) in DU145 cells. Data are means ± SEM (n=3). *P<0.05, **P < 0.01 vs TGF-β 0h (Student's t-test). (E) Analysis the expression of SPOP in the treatment of TGF-β (10ng/ml) in LNCaP cells. Data are means ± SEM (n=3). *P<0.05, **P < 0.01 vs TGF-β 0h (Student's t-test).