Research Paper Volume 12, Issue 20 pp 20069—20084

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2

Figure 3. FAM181A-AS1 overexpression promotes cell proliferation and colony formation. (A) QRT-PCR analysis shows FAM181A-AS1 levels in pcDNA3.1- and pcDNA3.1-FAM181A-AS1-transfected U87 and U251 cell lines. (B, C) CCK-8 and EdU assay results show proliferation of control and FAM181A-AS1-overexpressing U87 and U251 cell lines. (D) Colony formation assay shows the total numbers of colonies formed by control and FAM181A-AS1-overexpressing U87 and U251 cell lines. (E) Western blot analysis shows the levels of PCNA protein in control and FAM181A-AS1-overexpressing U87 and U251 cell lines. Note: **P<0.01, ***P<0.001; data are represented as means ± SD.