Research Paper Volume 12, Issue 20 pp 20069—20084

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2

Figure 6. MiR-129-5p mimic treatment reduces cell proliferation and colony formation FAM181A-AS1-overexpressing glioma cell lines. (A, B) CCK-8 and EdU assay results show proliferation of FAM181A-AS1-overexpressing U87 and U251 cell lines transfected with miR-NC (negative control) or miR-129-5p mimic. (C) Colony formation assay results show the total numbers of colonies formed by FAM181A-AS1-overexpressing U87 and U251 cell lines transfected with miR-NC (negative control) or miR-129-5p mimic. Note: **P<0.01, ***P<0.001; ns, not statistically significant; data are represented as means ± SD.