Research Paper Volume 13, Issue 2 pp 1842—1858

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma

DAPK1 overexpression in the sunitinib-resistant ccRCC cells reduces the growth of xenograft tumors in the nude mice model. (A, B) Western blot analysis shows DAPK1 protein expression in parental (786-O and ACHN) and sunitinib-resistant (786O-R and ACHN-R) ccRCC cells. GAPDH is used as internal control. (C) CCK-8 assay results show the growth rate of control-ACHN-R, DAPK1-overexpressing ACHN-R, and DAPK1-knockdown ACHN-R cells that are treated with different concentrations of sunitinib. (D) CCK-8 assay results show the growth rate of control and DAPK1 overexpressing 786O-R cells that are treated with different concentrations of sunitinib. The picture (E), average weight (F) and volume (G) of the tumors obtained after 7 weeks of transplanting control and DAPK1 overexpressing 786O-R cells in the nude mice. (H) Immunohistochemical analysis of the xenograft tumors obtained by subcutaneously transplanting control and DAPK1 overexpressing 786O-R cells in nude mice. The bar graphs show the statistical analysis of three independent experiments. Note: The data are shown as means ± SEM. ***p

Figure 6. DAPK1 overexpression in the sunitinib-resistant ccRCC cells reduces the growth of xenograft tumors in the nude mice model. (A, B) Western blot analysis shows DAPK1 protein expression in parental (786-O and ACHN) and sunitinib-resistant (786O-R and ACHN-R) ccRCC cells. GAPDH is used as internal control. (C) CCK-8 assay results show the growth rate of control-ACHN-R, DAPK1-overexpressing ACHN-R, and DAPK1-knockdown ACHN-R cells that are treated with different concentrations of sunitinib. (D) CCK-8 assay results show the growth rate of control and DAPK1 overexpressing 786O-R cells that are treated with different concentrations of sunitinib. The picture (E), average weight (F) and volume (G) of the tumors obtained after 7 weeks of transplanting control and DAPK1 overexpressing 786O-R cells in the nude mice. (H) Immunohistochemical analysis of the xenograft tumors obtained by subcutaneously transplanting control and DAPK1 overexpressing 786O-R cells in nude mice. The bar graphs show the statistical analysis of three independent experiments. Note: The data are shown as means ± SEM. ***p<0.001, ** p<0.01, *p<0.05.