Figure 3. Lentiviral vectors were used to overexpress or silencing LGALS1 using 3 shRNAs. Western blot analysis showing decreased GAL-1 protein expression in (A) MGC-803 cells, and (C) AGS cells transfected with LGALS1 shRNAs, compared with cells transfected with control shRNA (shCtl). Western blot showing GAL-1 protein expression following overexpression of LGALS1 (OE-LGALS1) compared to the empty vector (OE-Con) in (B) MGC-803 cells, and (D) AGS cells. Reduced expression levels of LGALS1 mRNA in (E) MGC-803 cells, and (G) AGS cells transfected with LGALS1 shRNAs. Increased expression levels of LGALS1 with OE-LGALS1 in (F) MGC-803 cells, and (H) AGS cells transfected with OE-LGALS1. GAPDH was used as a loading control. (All n = 3, *P < 0.05, **P < 0.01).