Figure 4.LGALS1 promotes GLI1 expression in vitro. (A and C) Western blotting indicating that OE- LGALS1 efficiently induced GLI1 protein expression compared with wild-type control and negative control-transduced cells. (B and D) Transfection with shLGALS1#3 inhibited GLI1 protein expression compared with wild-type control and negative control-transduced cells. These results were confirmed at the mRNA level by qRT-PCR (E–H). GAPDH served as a loading control. (All n = 3, *P < 0.05, **P < 0.01).
