Research Paper Volume 12, Issue 22 pp 23082—23095

Deubiquitinase USP7 regulates Drosophila aging through ubiquitination and autophagy

Figure 5. Effect of dusp7 in Drosophila on the control of protein in vivo. (A) The protein ubiquitination level after dusp7 knockdown in Drosophila. (B) The ubiquitination level after dusp7 knockdown in Drosophila (* p<0.05). (C) Autophagy-related gene expression after dusp7 knockdown in Drosophila (* p<0.05). (D) Autophagy-related protein expression after dusp7 knockdown in Drosophila (** p<0.01). (E) The effect of autolysosomes after dusp7 knockdown in Drosophila (*** p<0.001; white arrow points to autophagosome). (>: hybridization; + means adding Ru to induce expression; - means no adding RU).