Research Paper Advance Articles pp 24270—24287

Metformin protects against myocardial ischemia-reperfusion injury and cell pyroptosis via AMPK/NLRP3 inflammasome pathway

Figure 4. The inhibiting effects of Metformin on NLRP3 inflammasome activation within the infarct area following myocardial I/R injury. (A) Apoptosis-related proteins in the ischemic area, including NLRP3, ASC, cleaved-caspase 1, IL-1β, IL-18 and GAPDH were examined by Western blot analysis. (BF) Quantitative analysis of NLRP3, ASC, cleaved-caspase 1, IL-1β and IL-18 expression (n = 4 per group). (G) Bottom representative immunohistochemical-stained NLRP3 in cardiac tissue of each group are shown (n = 4 per group). Magnification 200x, Scale bar = 100 μm; Values are expressed as the mean ± SEM. * P < 0.05 vs. Sham. # P < 0.05 vs. I/R. & P < 0.05 vs. MET.