Research Paper Volume 12, Issue 21 pp 22291—22312

LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA

Figure 4. MiR-194-5p acted as the antioncogene. The relative expression level of miR-194-5p was decreased by miR-194-5p inhibitor and increased by miR-194-5p mimics (A). Cell proliferation was detected in both BC cells after the transfection of miR-194-5p inhibitor or mimics (B, C). Representative images of EdU assay and the relative fold changes of EdU positive cells were detected by miR-194-5p inhibitor or mimics (D, E). The relative cell migration was restrained or accelerated after the transfection of miR-194-5p mimics or inhibitor in the T24 and UM-UC-3 (F, G) cell lines. Apoptotic cells were measured after the transfection of miR-194-5p mimics or inhibitor in the T24 and UM-UC-3(H, I) cell lines by flow cytometry analysis. (*P < 0.05, **P < 0.01).