Research Paper Volume 12, Issue 21 pp 22291—22312

LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA

Figure 5. PVT1 was a target of miR-194-5p. The relative expression of miR-194-5p was increased by si-PVT1 (A). Dual-luciferase reporter assays were performed in T24 or UM-UC-3 cells co-transfected with PVT1-Wt or PVT1-Mut and miR-194-5p mimics or NC (B). Cell proliferation was detected in both BC cell lines after co-transfection with si-NC+NC, si-PVT1+miR-194-5p inhibitor or si-PVT1+miR-194-5p mimics (C, D). The relative cell migration after the co-transfection with si-NC+NC, si-PVT1+miR-194-5p inhibitor or si-PVT1+miR-194-5p mimics, and the representative images were as follow (E, F). The apoptotic cells were measured after the co-transfection with si-C+NC, si-PVT1+miR-194-5p inhibitor or si-PVT1+miR-194-5p mimics by flow cytometry analysis (G, H). (*P < 0.05, **P < 0.01).