Research Paper Volume 12, Issue 21 pp 22291—22312

LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA

Figure 8. MiR-194-5p positively regulates BCLAF1 expression. The relative expression level of miR-194-5p was reduced by miR-194-5p inhibitor in SV-HUC-1 (A). Cell proliferation was detected in SV-HUC-1 after transfection of miR-194-5p inhibitor (B). The relative cell migration was accelerated after transfection of miR-194-5p inhibitor in the SV-HUC-1 (C). Apoptotic cells were measured after transfection of miR-194-5p inhibitor in SV-HUC-1 (D). Cell proliferation was detected in both bladder carcinomas cell lines after co-transfection with si-NC+NC and si-BCLAF1+miR-194-5p inhibitor or mimics (E, F). The relative cell migration after co-transfection with si-NC+NC, si-BCLAF1+miR-194-5p inhibitor or mimics, and the representative images were as follow (G, H). The apoptotic cells were measured after co-transfection with si-C+NC, si-BCLAF1+miR-194-5p inhibitor or mimics by flow cytometry analysis (I, J). (*P < 0.05, **P < 0.01).