Research Paper Advance Articles pp 23525—23547

Mutation in histone deacetylase HDA-3 leads to shortened locomotor healthspan in Caenorhabditis elegans

Figure 2. HDA-3 G271E missense mutation leads to progressive decline in locomotor ability. (A) (Left) Sequence of hda-3(ix260) allele which is the same amino acid sequence as WT. (Right) Sequence of hda-3(ix261) allele which is the same amino acid sequence as the hda-3(ix241) allele. Both sequences carry a synonymous mutation site to disrupt the PAM sequence to prevent repetitive editing by CRISPR-Cas9. (B) (Left) Maximum velocities of WT, OF1263 hda-3(ix241);dys-1(ix259) (4x BC), OF1353 hda-3(ix260);dys-1(ix259) and OF1354 hda-3(ix260);dys-1(ix259) worms. (Right) Percent change in maximum velocity of WT, OF1263, OF1353 and OF1354 worms on adult day 5 compared to adult day 1. (C) (Left) Maximum velocities of WT, OF1355 hda-3(ix261), OF1356 hda-3(ix261) worms. (Right) Percent change in maximum velocity of WT, OF1355 hda-3(ix261), OF1356 hda-3(ix261) worms on adult day 5 compared to adult day 1. (D) (Left) Maximum velocities of WT and hda-3(ok1991) worms. (Right) Percent change in maximum velocity of WT and hda-3(ok1991) worms on adult day 5 compared to adult day 1. For maximum velocity measurements, N = 30–45 worms per strain for each day (10–15 worms from 3 biological replicate plates). For percent change in maximum velocity graphs, N = 3 biological replicate plates. ***p < 0.001; **p < 0.01; *p < 0.05.