Research Paper Volume 13, Issue 2 pp 2668—2680

Notch-1 promotes the malignant progression of osteosarcoma through the activation of cell division cycle 20

Notch-1 shRNA alleviated Cdc20 expression. (A) Wound healing assays showed that Notch-1 shRNA moderated wound closure. (B) Quantitative analysis f the migration data. *P C) Real-time RT-PCR results showing Notch-1, Cdc20, Bim and p21 expression. (D) Western blot results showing Notch-1, Cdc20, Bim and p21 expression.

Figure 2. Notch-1 shRNA alleviated Cdc20 expression. (A) Wound healing assays showed that Notch-1 shRNA moderated wound closure. (B) Quantitative analysis f the migration data. *P < 0.05 vs control shRNA. (C) Real-time RT-PCR results showing Notch-1, Cdc20, Bim and p21 expression. (D) Western blot results showing Notch-1, Cdc20, Bim and p21 expression.