Research Paper Volume 13, Issue 2 pp 2668—2680

Notch-1 promotes the malignant progression of osteosarcoma through the activation of cell division cycle 20

Notch-1 overexpression elevated Cdc20 expression. (A) The migratory ability was promoted by Notch-1 overexpression. (B) Quantification of the migration analysis results. *P C) Real-time RT-PCR showing Notch-1, Cdc20, p21 and Bim expression in cells with Notch-1 upregulation. (D) Western blot results showing Notch-1, Cdc20, p21 and Bim expression in cells with Notch-1 upregulation.

Figure 4. Notch-1 overexpression elevated Cdc20 expression. (A) The migratory ability was promoted by Notch-1 overexpression. (B) Quantification of the migration analysis results. *P < 0.05 vs control cDNA. (C) Real-time RT-PCR showing Notch-1, Cdc20, p21 and Bim expression in cells with Notch-1 upregulation. (D) Western blot results showing Notch-1, Cdc20, p21 and Bim expression in cells with Notch-1 upregulation.