Research Paper Volume 13, Issue 12 pp 16072—16087

MiR-513b-5p represses autophagy during the malignant progression of hepatocellular carcinoma by targeting PIK3R3

PIK3R3 is targeted by miR-513b-5p in liver cancer cells. (A) The interaction prediction analysis of miR-513b-5p with PIK3R3 mRNA 3′UTR using ENCORI online database. (B–E) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic. (B and C) The analysis of luciferase activities using luciferase reporter gene assays. (D and E) The analysis of PIK3R3 mRNA expression using qPCR. (F) The detection of PIK3R3 expression using Western blot analysis.

Figure 5. PIK3R3 is targeted by miR-513b-5p in liver cancer cells. (A) The interaction prediction analysis of miR-513b-5p with PIK3R3 mRNA 3′UTR using ENCORI online database. (BE) The HepG2 and Huh-7 cells were treated with miR-513b-5p mimic. (B and C) The analysis of luciferase activities using luciferase reporter gene assays. (D and E) The analysis of PIK3R3 mRNA expression using qPCR. (F) The detection of PIK3R3 expression using Western blot analysis.