Figure 2. (A) Alizarin Red S staining showed mineral deposits in HA-VSMCs under different treatments. (B) Determination of the Ca2+ concentration and AKP activity in each group. (C, D) RT-qPCR analysis of mRNAs associated with osteogenic transdifferentiation. (E, F) Western blot analysis of protein expression. *P<0.05 compared with the NC group; #P<0.05 compared with the Pi group.