Research Paper Volume 13, Issue 13 pp 17274—17284

Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression

Circ

Figure 1. Circ_0081001, miR-494-3p, and BACH1 were potential regulators in OS. (A) Volcano map was used to indicate the difference of the expression levels of miRNAs between normal bone tissues and OS tissues in GSE28423, and the upregulated and downregulated miRNAs expressions were marked with red and green, respectively. (B) Venn diagram showed the intersection of predicted miRNA targets of circ_0081001 and lowly expressed miRNAs in OS tissues in GSE28423. (C) MiRmap, microT, miRanda, and TargetScan were employed for predicting the downstream target genes of miR-494-3p. (D) The potential biological function of miR-494-3p was predicted utilizing DAVID GO enrichment analysis. (E) Volcano map was used to indicate the difference of the gene expression profile between human normal osteoblasts and OS cell line in GSE11414, and the upregulated and downregulated gene expressions were marked with red and green, respectively. (F) Venn diagram showed the intersection of predicted target genes of miR-494-3p and highly expressed genes in OS tissues in GSE11414.