Research Paper Volume 13, Issue 13 pp 17274—17284

Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression

The targeted relationship between circ

Figure 4. The targeted relationship between circ_0081001 and miR-494-3p. (A) The potential binding site between miR-494-3p and circ_0081001 was predicted by bioinformatics analysis, and the binding site between miR-494-3p and circ_0081001 was shown. (B) qRT-PCR was implemented for examining the transfection efficiency of miR-494-3p mimics and miR-494-3p inhibitors. (C) The effects of miR-494-3p on circ_0081001 expression in OS cells were examined with qRT-PCR. (D) Dual-luciferase reporter gene experiment was employed for examining the targeted relationship between circ_0081001 and miR-494-3p. (E) RIP assay was performed for validating the direct interaction between circ_0081001 and miR-494-3p. (F) The impacts of circ_0081001 on miR-494-3p expression in OS cells were detected by qRT-PCR. ** and *** represent P<0.01 and P<0.001, respectively. ns represents P>0.05.