Figure 1. ANRIL and HMGB1 are over-expressed in pancreatic cancer. (A) qRT-PCR and western blot analyzed the expression of ANRIL, HMGB and miR-181a in human pancreatic cancer and adjacent tissues (n = 5). (B) qRT-PCR and (C) western blot analyzed the expression of ANRIL, HMGB and miR-181a in a series of human pancreatic cancer cell lines (PANC-1, ASPC-1, HPAC, BxPC-3) and normal pancreatic cells (HPDE). Standardized data with GAPDH. Student’s t test and analysis of variance compared the difference in A, B. *P < 0.05, **P < 0.01, ***P < 0.001.