Figure 1. Lipopolysaccharide (LPS) induces a dose- and time-dependent induction of the senescence-associate secretory phenotype (SASP) gene expression in non-senescent HUVECs (NC HUVEC) and ionizing radiation (IR)-induced senescent HUVECs (IR HUVEC). (A–F) Dose response. Relative gene expression of IL6 (A), IL1β (B), CCL2 (C), TNFα (D), CCL5 (E), and CXCL1 (F) in NC HUVEC and IR HUVEC stimulated with 3-100 ng/ml LPS for 3 hours. (G–L), Time course. Relative gene expression of IL6 (G), IL1β (H), CCL2 (I), TNFα (J), CXCL1 (K), and CCL5 (L) in NC HUVEC and IR HUVEC as a function of time of stimulation with 30ng/mL LPS. Gene expression in unstimulated NC HUVEC was used as baseline and GAPDH was used as endogenous control. (n = 3; mean ± SEM; * p<0.05, # p <0.01, + p<0.001 vs. non-SnC).