Research Paper Volume 13, Issue 15 pp 19878—19893

CCL2 associated with CD38 expression during ex vivo expansion in human cord blood-derived hematopoietic stem cells

(A) RT-qPCR analysis for mRNA expression of CCL2, TNFα, and FOS in serum and serum-free cultured hematopoietic stem cells. Quantification of gene expression normalized with respect to GAPDH. (n = 3, data are expressed as means ± SD. *p B) Western blot analysis for protein expression of CCL2, TNFα, and FOS in serum and serum-free cultured hematopoietic stem cells. GADPH was used as a loading control.

Figure 5. (A) RT-qPCR analysis for mRNA expression of CCL2, TNFα, and FOS in serum and serum-free cultured hematopoietic stem cells. Quantification of gene expression normalized with respect to GAPDH. (n = 3, data are expressed as means ± SD. *p < 0.05 versus serum free condition). (B) Western blot analysis for protein expression of CCL2, TNFα, and FOS in serum and serum-free cultured hematopoietic stem cells. GADPH was used as a loading control.