Research Paper Volume 13, Issue 17 pp 21155—21190

Liver-specific over-expression of Cripto-1 in transgenic mice promotes hepatocyte proliferation and deregulated expression of hepatocarcinogenesis-related genes and signaling pathways

Liver-specific overexpression of CR-1 gene in transgenic mice activates HCC-related signaling pathways. (A) Representative western blot images show expression levels of the indicated proteins in CR-1-overexpressing, CR-1-knockdown, and corresponding control BEL-7402 and HepG2 cells. (B) Representative immunoblotting images show levels of the indicated proteins in the liver tissues of Alb-Cre and RCLG/Alb-Cre mice. (C) QRT-PCR analysis shows relative mRNA levels of IL-1, IL-6, Notch1, and TGF-β1 in the liver tissues of Alb-Cre and RCLG/Alb-Cre mice.

Figure 5. Liver-specific overexpression of CR-1 gene in transgenic mice activates HCC-related signaling pathways. (A) Representative western blot images show expression levels of the indicated proteins in CR-1-overexpressing, CR-1-knockdown, and corresponding control BEL-7402 and HepG2 cells. (B) Representative immunoblotting images show levels of the indicated proteins in the liver tissues of Alb-Cre and RCLG/Alb-Cre mice. (C) QRT-PCR analysis shows relative mRNA levels of IL-1, IL-6, Notch1, and TGF-β1 in the liver tissues of Alb-Cre and RCLG/Alb-Cre mice.