Research Paper Volume 13, Issue 15 pp 19908—19919

A circular RNA, circSMARCA5, inhibits prostate cancer proliferative, migrative, and invasive capabilities via the miR-181b-5p/miR-17-3p-TIMP3 axis

circSMARCA5 suppresses tumor proliferative and invasive potential in vivo. Wild-type and circSMARCA5-overexpressed DU145 cells were injected into nude recipient mice. Every week, information about tumor size was collected. The tumor tissues were removed and the weight was determined four weeks after injection. Representative mice and resected tumors images (A, B). (C, D) Tumor weight and volume. (E) IHC staining of EMT markers, including E-cadherin, vimentin, and N-cadherin. Magnification ×20. (F) H&E staining and lung microscopic nodules quantification of each group. Magnification ×20. *, **, *** represent p ≤ 0.05, p ≤ 0.01, p ≤ 0.001, respectively.

Figure 4. circSMARCA5 suppresses tumor proliferative and invasive potential in vivo. Wild-type and circSMARCA5-overexpressed DU145 cells were injected into nude recipient mice. Every week, information about tumor size was collected. The tumor tissues were removed and the weight was determined four weeks after injection. Representative mice and resected tumors images (A, B). (C, D) Tumor weight and volume. (E) IHC staining of EMT markers, including E-cadherin, vimentin, and N-cadherin. Magnification ×20. (F) H&E staining and lung microscopic nodules quantification of each group. Magnification ×20. *, **, *** represent p ≤ 0.05, p ≤ 0.01, p ≤ 0.001, respectively.